By Shirley J. Gee
Content material:
Executive summary
, Pages v-vii
Acknowledgments
, Page viii
Preface
, Page ix
Section 1 - Introduction
, Pages 1-6
Section 2 - getting ready the laboratory
, Pages 7-8
Section three - Laboratory considerations
, Pages 9-13
Section four - Immunoassay tutorials for chosen environmental analytes
, Pages 14-53
Section five - Tutorials for aid techniques
, Pages 54-85
Section 6 - thesaurus of normal phrases in Immunoassay
, Pages 86-90
Appendix I - functionality insurance for air displacement pipettes
, Pages 91-102
Appendix II - functionality log and function try out worksheets for air displacement pipettors
, Pages 103-106
Abbreviations and acronyms
, Page 107
Read or Download Environmental Immunochemical Analysis Detection of Pesticides and Other Chemicals. A User's Guide PDF
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Extra info for Environmental Immunochemical Analysis Detection of Pesticides and Other Chemicals. A User's Guide
Sample text
The solvent was evaporated and the residue taken up in methanol, then diluted 1/10 with PBS-Tween/Azide. This extract only needed to be diluted 1/25 to eliminate matrix interferences. This assay can be used when samples contain up to 10% methanol. , 1993). Interference by a known media, such as 25% methanol can be corrected by running the standard curve in the equivalent media. This may compromise the parameters of the standard curve compared to running the standard curve in buffer, but the change is reproducible.
It should be stored in the refrigerator. Periodically tests of enzyme activity should be run to assure no loss of activity on storage of the stock solution. Working dilutions should be made up immediately before use and the extra discarded. DO NOT FREEZE - each freeze-thaw cycle will kill a significant part of the conjugate-enzyme activity. A change in the assay performance parameters will be an indication of possible degradation of the hapten-enzyme conjugate. Provided by Dr. Bruce Hammock, Department of Entomology, University of California, Davis, CA 95616.
E. Sigma Catalog No. e. Sigma Catalog No. 104-105, 5 mg tablets or equivalent) Safety Considerations: Read the Materials Safety Data Sheet (MSDS) provided by the manufacturer for any compound with which you are not familiar. Sodium azide is a teratogen; avoid breathing vapors or skin contact. It is assumed the analyst will have in place procedures for the safe handling of organic solvents and samples containing the analyte. q and Disposah The analyst should already have in place procedures for the disposal of organic solvents and samples containing the analyte.
