By David M Prescott
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Extra info for Methods in cell biology. / Volume XIII
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A . Ultrastructure . . . . . B. Carbohydrate Metabolism . . . . C . Lipid Metabolism . . . . D . Protein and Nucleic Acid Metabolism . . . E. Other Properties . . . . IX . I n VitroCulture of Parenchymal Liver Cells . . . X . Preparation of Nonparenchymal Liver Cells . . . References . . . . . 29 . . . . . . . . . 30 30 32 33 33 34 35 35 35 . 3 6 . 39 . 44 . 45 . 49 . 49 . 50 . . . . . . . . . . . . . . . . . . . . . . SEGLEN I. Introduction The liver occupies a central position in body metabolism, and its size, softness, and relative homogeneity have made it a favorite organ for biochemical investigation.
X . Preparation of Nonparenchymal Liver Cells . . . References . . . . . 29 . . . . . . . . . 30 30 32 33 33 34 35 35 35 . 3 6 . 39 . 44 . 45 . 49 . 49 . 50 . . . . . . . . . . . . . . . . . . . . . . SEGLEN I. Introduction The liver occupies a central position in body metabolism, and its size, softness, and relative homogeneity have made it a favorite organ for biochemical investigation. For the study of intact liver functions under controlled conditions the isolated, perfused rat liver has been extensively used.
14. Dependence of respiration on perfusate flow. 0 (0) at various flow rates. FromSeglen (1972a) with permission. , 5 ml per minute and use a technically simple open-flow(nonrecircu1ating) perfusion technique. This has been done by Capuzzi et al. (197 1, 1974) and Quistorff et al. (1973), and the results were reported to be satisfactory. In our experience, it is difficult to achieve uniform perfusion of the tissue at such a low flow rate, and intermittent short pulses of rapid flow work better in this respect.
