By Shelley D. Minteer
Hands-on researchers evaluate the rules at the back of winning miniaturization and describe the major strategies for miniaturizing large-scale biochemical and bioanalytical equipment for microchip research. The authors disguise not just the most well-liked equipment for the fabrication of microchips (photolithography, laser ablation, and tender lithography), but additionally microfluidic options for such bioanalytical assays and bioprocesses as DNA research, PCR, immunoassays, and mobilephone reactors. Highlights contain PCR on a microchip, microscale cellphone culturing, and the learn of mobile approaches on a microchip. The protocols supply step by step laboratory directions, an creation outlining the foundations at the back of the procedure, lists of the mandatory apparatus and reagents, and pointers on troubleshooting and fending off recognized pitfalls.
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Extra resources for Microfluidic Techniques: Reviews And Protocols
Example text
A cork borer is utilized to cut circles in the PDMS to form wells. The wells are cut before placing of the PDMS on the ablated sample. This method allows one to form a reversible seal of PDMS and polymethyl methacrylate. The second method involves placing an identical section of 1 × 3 in. of polymer on top of the ablated substrate and placing the pair under pressure and low heat approaching the glass transition temperature. The combination of heat and pressure permanently seals the device. 4. Notes 1.
7. Initiate the ablation process. Make note of the local atmosphere; that is, is the ablation occurring under an inert gas such as nitrogen, in a vacuum, or under a solvent such as methanol or water (see Notes 4–6). 8. After micromachining of the piece has been completed, ensure that the laser has stopped firing before attempting to remove the plastic. After removing the plastic from the stage, sonicate in a solution of 50:50 ethanol:water in order to remove any particulates that may remain on the surface.
Process flow for fabrication of gold microlectrodes using lift-off technique. Fabrication of Microelectrodes Using Lift-Off 25 4. Chrome or titanium (evaporation material) (see Note 2). 5. Shipley Microposit® SC 1827 positive photoresist (MicroChem, Newton, MA) (see Note 3). 6. Photoresist developer: Microposit MF 319 or Microposit MF 351 (MicroChem). 7. Acetone. 3. 1. Mask Fabrication Drawing and computer-aided design (CAD) programs such as Macromedia Freehand® and Autodesk AutoCAD® can be used to design masks for the fluidic device.
