By Peter J. Hollenbeck, James R. Bamburg
This publication lays out a number of uncomplicated suggestions for becoming and engaging in experiments with many sorts of neurons. topics contain peripheral and principal neurons from vertebrate and invertebrate resources, in addition to neuron-like phone strains. It additionally explains fresh advances in our skill to introduce exogenous proteins and genes to neurons in tradition. tactics for winning protein infiltration, biolistic transfection, electroporation, and viral transgenic tools in neurons also are awarded. * includes tradition method for greater than a dozen sorts of CNS and PNS neurons * contains newest and trustworthy strategies from specialist practitioners for particular experimental functions* Addresses the most recent techniques for transfecting neurons
Read Online or Download Neurons: Methods and Applications for the Cell Biologist PDF
Best nonfiction_3 books
Night of Ghosts and Lightning (Planet Builders, No. 2)
E-book by way of Tallis, Robyn
Additional resources for Neurons: Methods and Applications for the Cell Biologist
Sample text
D. Ease and Cost of Obtaining and Growing Neurons For many research questions in cell biology, there is likely to be more than one suitable neuronal culture system. If this is the case, then it only makes sense to use the one that is the least expensive to purchase, least expensive to house, easiest or least destructive to obtain, and easiest to grow and handle. This is an area in which neuronal cell lines look very attractive (Chapters 12 and 13). The following areas are worth considering (Table IV).
Cultures of this kind are used far less commonly than dissociated cultures because the morphology of individual neurons cannot be fully discerned. However, there are special applications for which explant cultures are ideally suited. For example, explant cultures can be used for biochemical analyses in which one wishes to compare composition of the axons with that of cell bodies and dendrites. The cell body mass can simply be cut away from the axons under a dissecting microscope and then plucked out of the culture after development of the axonal halo.
B) The two large salivary glands (one of which is outlined by arrowheads) along the midline were exposed after the neck skin was cut open. (C) After the salivary glands were removed, the sternocleidomastoid muscle (bracketed by arrows) was revealed on both sides. (D) Transection of the sternocleidomastoid muscle and strap muscles beneath has exposed the carotid artery and its branch (shown at higher magnification). The nodose ganglion (indicated by the arrowhead) is located directly lateral to the branch point of the carotid artery, and the sympathetic ganglion (indicated by the arrow) is located directly beneath the branch point.
