By Ramón Sánchez-Ocaña
El sobrepeso es un problema que afecta tanto a los angeles salud como a los angeles autoestima de millones de personas. Ramón Sánchez-Ocaña, famoso periodista experto en temas de salud, describe en este libro las dietas más populares y analiza las ventajas e inconvenientes de cada una de ellas. Además, te explica con su estilo claro y ameno cómo funciona el cuerpo humano ycuáles son sus necesidades energéticas básicas, qué es el metabolismo y qué tratamientos existen para combatir l. a. obesidad.
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Example text
Waterproof Activated Cysteines Make Transglutaminases – In Theory Cysteine proteases, the prototype of which is papain, are phylogenetic relatives of the ‘classical’ (or, more exactly ‘nominal’) transglutaminases (TGases). TGases differ from them mainly in the rate of access by water or by other acyl-acceptor nucleophiles to the catalytic site. If water has free access to the acyl-enzyme thioester intermediate, other substrates have little chance to compete with the abundance and nucleophilicity of this solvent.
The catalytic activities of TGase 6 and TGase 7 have not yet been demonstrated. Regulators of TGases Owing to the potentially disruptive effects of glutamine deamidation and transamidation, human transglutaminases are expressed and stored in zymogenic or otherwise inactivated forms and can be activated only in the presence of calcium levels which are not common and ubiquitous in living cells. TGase 1, TGase 3 and factor XIIIa, which typically function in highcalcium milieus of keratinocytes and blood plasma, are expressed and stored as zymogens and require enzymatic cleavage besides Ca2ϩ to attain active conformation.
Further, we identify these proteins as ‘intracellular’ or ‘extracellular’ in order to facilitate a correct framing of the overall functions, at least at intra- or extra-cellular level. In some cases, it was possible to report also the subcellular localization for such substrate. Of course, protein secretion or translocation processes may move proteins from a compartment to another under particular conditions. Further, the peculiar localization of a TGase isoform within a specific compartment, like for instance the TGase 2 in cytosolic compartment and FXIII in plasmatic fluid, is today not more accepted as a dogmatic rule, since their localization in other compartments was demonstrated in several reports [13–16].
