By Padma Viswanathan
Sivakami used to be married at ten, widowed at eighteen, and left with youngsters. based on the dictates of her caste, her head is shaved and he or she places on widow's whites. From sunrise to nightfall, she isn't really allowed to infect herself with human contact, no longer even to convenience her young children. Sivakami dutifully follows customized, apart from one defiant act: She strikes again to her lifeless husband's condo to elevate her childrens. There, her servant Muchami, a closeted homosexual guy who's sure through a distinct caste's ideas, turns into her public face. Their singular courting holds 3 generations of the relatives jointly throughout the turbulent first half the 20th century, as India endures nice social and political swap. yet as time passes, the relatives alterations, too; Sivakami's son will query the strictures of the very ideals that his mom has scrupulously upheld. The Toss of a Lemon is heartbreaking and exhilarating, profoundly unique but totally recognizable in evoking the tensions that modify brings to each family members.
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5). Hypothetically, one of these pathways includes removal of the propeptide, leading to processing of carbohydrates into some complex forms, while the other one involves protein with retained propeptide resulting in conservation of high mannose oligosaccharides only (Fig. 5). 28 Urban Gullberg et al. S Is Sorting Dependent on Processing? Constitutive secretion of both lysosomal and azurophil granule proteins seems to be limited to unprocessed proforms. Therefore, recognition for sorting to granules could be specific for processed forms and processing would then be a prerequisite for sorting to granules.
A 125 amino acid aminoterminal propeptide, a small peptide between the light and heavy chains, as well as a single serine residue of the carboxy terminus are each excised during processing of proMPO into mature MPO. Similar to the maturation of serine proteases, proteolytic processing occurs in post-endoplasmic reticulum (post-ER) compartments, but mature MPO has been found to be sensitive to digestion with endoglycosidase H (Endo H), indicating Urban Gullberg et al. 26 - light heavy propeptide subunit ((3) subunit (11) Translocation into ER Cleavage of signal peptide signal peptide ERI Goigi Acquisition of N·linked carbohydrates which are partially processed into complex forms oli osaccharides [.
Autocatalytic cleavage of MPO between Met 409 and 6 4 5 1 2 3 Pro 410 due to the presence of the sulfonium linkage. Natural, recombinant and mutant MPO (5 g) were dissolved in sample buffer without beta-mercapto94 - ethanol and heated at 90°C for 67 _ 8 min, and analyzed by SDSPAGE. Lane 2 the heating of natural MPO resulted in a frag43 ment of 38 kDa. 1 with the light and heavy subunit. Lane 4 the autolytic cleavage was not observed in the mutant Met 409Gln. Lane 5 as a control, this cleavage occurred in the close mutant Glu 408Gln.
